Product Info Summary
SKU: | PB9207 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™
View all Glutamate Receptor 4 Antibodies
SKU/Catalog Number
PB9207
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™ catalog # PB9207. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9207)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GRIA4 recombinant protein (Position: Q176-K350). Human GRIA4 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9207 is reactive to GRIA4 in Human, Mouse, Rat
Applications
PB9207 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
101 kDa
Calculated molecular weight
100.871kDa
Background of Glutamate Receptor 4
Glutamate receptor 4 also named GRIA4 or GLUR4, is a protein that in humans is encoded by the GRIA4 gene. It is mapped to 11q22.3. This gene is a member of a family of L-glutamate-gated ion channels that mediate fast synaptic excitatory neurotransmission. These channels are also responsive to the glutamate agonist, alpha-amino-3-hydroxy-5-methyl-4-isoxazolpropionate (AMPA). Some haplotypes of this gene show a positive association with schizophrenia. What's more, glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GRIA4 using anti-GRIA4 antibody (PB9207).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: Mouse Brain Tissue Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA4 antigen affinity purified polyclonal antibody (Catalog # PB9207) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA4 at approximately 101KD. The expected band size for GRIA4 is at 101KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of A549 cells using anti-GRIA4 antibody (PB9207).
Overlay histogram showing A549 cells stained with PB9207 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA4 Antibody (PB9207,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For GRIA4 (Source: Uniprot.org, NCBI)
Gene Name
GRIA4
Full Name
Glutamate receptor 4
Weight
100.871kDa
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
AMPA-selective glutamate receptor 4; GluA4; GluR4; gluR-4; GLUR4C; GLUR4gluR-D; GLURD; GluR-D; glutamate receptor 4; Glutamate receptor ionotropic, AMPA 4; glutamate receptor, ionotrophic, AMPA 4 GRIA4 GLUR4, GLUR4C, GLURD, GluA4, NEDSGA glutamate ionotropic receptor AMPA type subunit 4 glutamate receptor 4|AMPA-selective glutamate receptor 4|gluR-4|gluR-D|glutamate receptor, ionotrophic, AMPA 4|glutamate receptor, ionotropic, AMPA 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIA4, check out the GRIA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™ (PB9207)
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No publications found for PB9207
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™
Question
Which GRIA4 region does this antibody target? Which part of the protein was used for generating the antibody? (I am particularly interested in the N-terminal region)
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
This antibody targets the E. Coli-derived human GRIA4 recombinant protein (Position: Q176-K350), and the entire sequence of Q176-K350 was used as the immunogen. Note: Human GRIA4 shares 99% amino acid sequence identity with mouse and rat GRIA4.
Boster Scientific Support
Answered: 2020-03-20
Question
See attached the WB image, lot number and protocol we used for corpus callosum using anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
We are currently using anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
The anti-Ionotropic Glutamate receptor 4/GRIA4 antibody (PB9207) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-01
Question
I was wanting to use your anti-Ionotropic Glutamate receptor 4/GRIA4 antibody for Flow Cytometry for rat corpus callosum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat corpus callosum identification?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
You can see on the product datasheet, PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody has been tested for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat corpus callosum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-27
Question
Does anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 work on goat Flow Cytometry with brain?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
Our lab technicians have not tested anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-21
Question
Has this antibody been validated for immunocytochemistry in rat hippocampal neuron?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
A; This antibody has been validated for western blot only, and not for ICC.
Boster Scientific Support
Answered: 2017-12-12
Question
Is this PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody reactive to the isotypes of GRIA4?
J. Edwards
Verified customer
Asked: 2016-04-06
Answer
The immunogen of PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody is E.coli-derived human GRIA4 recombinant protein (Position: Q176-K350). Human GRIA4 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-06
Question
Would PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Wu
Verified customer
Asked: 2014-08-13
Answer
As indicated on the product datasheet, PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-13