Anti-GRK3/ADRBK2 Antibody Picoband™

GRK3 antibody

Boster Bio Anti-GRK3/ADRBK2 Antibody Picoband™ catalog # PB9682. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9682
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

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Product Name

Anti-GRK3/ADRBK2 Antibody Picoband™

View all GRK3 Antibodies

SKU/Catalog Number

PB9682

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GRK3/ADRBK2 Antibody Picoband™ catalog # PB9682. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GRK3/ADRBK2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9682)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GRK3, different from the related mouse and rat sequences by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9682 is reactive to GRK3 in Human

Applications

PB9682 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

79.71kDa

Background of GRK3

Beta-adrenergic receptor kinase 2  (beta-ARK-2), also known as G-protein-coupled receptor kinase 3  (GRK3), is an enzyme that in humans is encoded by the ADRBK2 gene. The human ADRBK2 gene is located on 22q11. The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For GRK3 (Source: Uniprot.org, NCBI)

Gene Name

GRK3

Full Name

Beta-adrenergic receptor kinase 2

Weight

79.71kDa

Superfamily

protein kinase superfamily

Alternative Names

Beta-adrenergic receptor kinase 2 GRK3 ADRBK2, BARK2 G protein-coupled receptor kinase 3 beta-adrenergic receptor kinase 2|adrenergic, beta, receptor kinase 2|beta-ARK-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GRK3, check out the GRK3 Infographic

GRK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GRK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-GRK3/ADRBK2 Antibody Picoband™

Question

We are currently using anti-GRK3/ADRBK2 antibody PB9682 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

A. Banerjee

Verified customer

Asked: 2014-08-28

Answer

The anti-GRK3/ADRBK2 antibody (PB9682) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-08-28

Size

Conjugation

Total: $370

SKU:PB9682

In stock, 3+ left.

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PB9682
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

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