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Product Info Summary
| SKU: | M20057 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GRK3 Monoclonal Antibody
SKU/Catalog Number
M20057
BM5504 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GRK3 Monoclonal Antibody catalog # M20057. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRK3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M20057)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEBE-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GRK3 Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.
Reactive Species
M20057 is reactive to GRK3 in Human
Observed Molecular Weight
80 kDa
Calculated molecular weight
79.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M20057 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: Ramos cell
Validation Images & Assay Conditions
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Western blot analysis of GRK3 expression in Ramos cell lysate.
Specific Publications For Anti-GRK3 Monoclonal Antibody (M20057)
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Customer Q&As
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5 Customer Q&As for Anti-GRK3 Monoclonal Antibody
Question
Does anti-GRK3 Monoclonal antibody M20057 work for IF with brodmann (1909) area 23?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
According to the expression profile of brodmann (1909) area 23, GRK3 is highly expressed in brodmann (1909) area 23. So, it is likely that anti-GRK3 Monoclonal antibody M20057 will work for IF with brodmann (1909) area 23.
Boster Scientific Support
Answered: 2020-04-20
Question
I see that the anti-GRK3 Monoclonal antibody M20057 works with IF, what is the protocol used to produce the result images on the product page?
M. Mangal
Verified customer
Asked: 2019-10-22
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-22
Question
Would M20057 anti-GRK3 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
It shows on the product datasheet, M20057 anti-GRK3 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-05
Question
We are currently using anti-GRK3 Monoclonal antibody M20057 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
The anti-GRK3 Monoclonal antibody (M20057) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-26
Question
I was wanting to use your anti-GRK3 Monoclonal antibody for IF for human brodmann (1909) area 23 on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brodmann (1909) area 23 identification?
P. Collins
Verified customer
Asked: 2015-11-05
Answer
It shows on the product datasheet, M20057 anti-GRK3 Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brodmann (1909) area 23 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-05
