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Product Info Summary
| SKU: | M00851-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00851-1
BM5026 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody catalog # M00851-1. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00851-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AADG-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Growth Hormone
Reactive Species
M00851-1 is reactive to GH1 in Human
Observed Molecular Weight
22 kDa
Calculated molecular weight
24.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00851-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human placenta tissue
IHC: human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of GH1 using anti-GH1 antibody (M00851-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GH1 antigen affinity purified monoclonal antibody (Catalog # M00851-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 22 kDa. The expected band size for GH1 is at 25 kDa.
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Immunohistochemical analysis of paraffin-embedded human placenta, using Growth Hormone Antibody.
Specific Publications For Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody (M00851-1)
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Customer Q&As
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3 Customer Q&As for Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody
Question
What is the amino acid range used as the immunogen for M00851-1?
Verified customer
Asked: 2021-01-26
Answer
The amino acid sequence or range used as the immunogen for the Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody (M00851-1) is around aa 58-187.
Boster Scientific Support
Answered: 2021-01-26
Question
Can M00851-1 distinguish between the 22 kda and 20 kda forms of GH?
Verified customer
Asked: 2021-01-26
Answer
No, the Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody (M00851-1 can not distinguish the two different GH isoforms 20K and 22K.
Boster Scientific Support
Answered: 2021-01-26
Question
What is epitope of GH that is recognized by M00851-1?
Verified customer
Asked: 2021-01-25
Answer
We did not perform epitope mapping with these antibodies. The immunogen sequence for the Anti-Growth Hormone GH1 Rabbit Monoclonal Antibody (M00851-1) is ESIPTPSNREETQQK which is shared by isoforms 20K and 22K. The antibody cannot distinguish the two different GH isoforms 20K and 22K.
Boster Scientific Support
Answered: 2021-01-26
