Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody

Product Name

Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody

View all gp96/HSP90B1/GRP94 Antibodies

SKU/Catalog Number

M02393

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody (Catalog # M02393). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea pig, Chicken, Xenopus laevis.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02393)

Host

Rat

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM249

Isotype

IgG2a, kappa

Immunogen

Purified glucose regulated protein 94 (grp94) from chicken oviducts

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02393 is reactive to HSP90B1 in Chicken, Cow, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Horse, Xenopus laevis

Applications

M02393 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

92.469kDa

Background of gp96/HSP90B1/GRP94

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This monoclonal antibody is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Immunofluorescence analysis of HePG2 cells labeling GRP94 with Anti-GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Anti-GRP94 Monoclonal Antibody (SPM249).

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Flow Cytometric analysis of PFA-fixed HePG2 cells using Anti-GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For HSP90B1 (Source: Uniprot.org, NCBI)

Alternative Names

94 kDa glucose-regulated protein; ECGP; Endoplasmin; endothelial cell (HBMEC) glycoprotein; glucose regulated protein, 94 kDa; gp96; GP96ECGP; Grp94; GRP-94; GRP94gp96 homolog; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; HSP90B1; TRA1endoplasmin; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen-1 (gp96) HSP90B1 ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1 heat shock protein 90 beta family member 1 endoplasmin|94 kDa glucose-regulated protein|endothelial cell (HBMEC) glycoprotein|epididymis luminal protein 35|epididymis secretory sperm binding protein Li 125m|heat shock protein 90 kDa beta member 1|heat shock protein 90kDa beta (Grp94), member 1|heat shock protein 90kDa beta family member 1|stress-inducible tumor rejection antigen gp96|tumor rejection antigen (gp96) 1|tumor rejection antigen 1

For more info on HSP90B1, check out the HSP90B1 Infographic

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In this infographic, you will see the following information for HSP90B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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