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GSDMD antibody (M02842-1) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of GSDMD using anti-GSDMD antibody (M02842-1).
GSDMD was detected in a paraffin-embedded section of human esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-GSDMD Antibody (M02842-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
GSDMD was detected in a paraffin-embedded section of human esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-GSDMD Antibody (M02842-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti-GSDMD Antibody Picoband® (monoclonal, 3D5)
GSDMD antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IHC, WB |
Tested Applications
GSDMD antibody was positively detected by WB in:
human THP-1 whole cell, human SW620 whole cell, human Jurkat whole cell, human U87 whole cell, human PC-3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GSDMD antibody was positively detected by IHC in:
human esophagus tissue, human liver cancer tissue, human lymph nodes tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-GSDMD Antibody Picoband® (monoclonal, 3D5)
View all GSDMD Antibodies
View all GSDMD Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3D5
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human GSDMD recombinant protein (Position: M1-H484).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
53 kDa
Calculated Molecular Weight
52.8 kDa
Background of GSDMD
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Cite This Product
Anti-GSDMD Antibody Picoband® (monoclonal, 3D5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02842-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
88%
Moderate Homology
Cow
64%
Low homology
Sheep
62%
Low homology
Rabbit
60%
Low homology
Dog
60%
Low homology
Mouse
58%
Low homology
Rat
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GSDMD antibody
Immunohistochemistry (IHC) protocol for GSDMD antibody
Publications
Application:
Species:
Penicilazaphilone C alleviates allergic airway inflammation and improves the immune microenvironment by hindering the NLRP3 inflammasome
Published: 2024-05-20
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 6.90
Species: Mouse
PMID: 38772153
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Product Promise
We promise Anti-GSDMD Antibody Picoband® (monoclonal, 3D5) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02842-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026