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GSDMD antibody (M02842) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of Jurkat cells using anti-GSDMD antibody (M02842).
Overlay histogram showing Jurkat cells stained with M02842 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSDMD Antibody (M02842, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Jurkat cells stained with M02842 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSDMD Antibody (M02842, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GSDMD Antibody Picoband® (monoclonal, 6D11)
GSDMD antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
GSDMD antibody was positively detected by WB in:
human THP-1 whole cell, human SW620 whole cell, human Jurkat whole cell, human U87 whole cell, human PC-3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GSDMD antibody was positively detected by IHC in:
human cervical cancern tissue, human cervical intraepithelial neoplasia tissue, human esophageal squamous carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GSDMD antibody was positively detected by ICC/IF in:
PC-3 cell
GSDMD antibody was positively detected by FC in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-GSDMD Antibody Picoband® (monoclonal, 6D11)
View all GSDMD Antibodies
View all GSDMD Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6D11
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human GSDMD recombinant protein (Position: M1-H484).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
53 kDa
Calculated Molecular Weight
52.8 kDa
Background of GSDMD
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Cite This Product
Anti-GSDMD Antibody Picoband® (monoclonal, 6D11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02842)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
88%
Moderate Homology
Cow
64%
Low homology
Sheep
62%
Low homology
Rabbit
60%
Low homology
Dog
60%
Low homology
Mouse
58%
Low homology
Rat
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GSDMD antibody
Immunohistochemistry (IHC) protocol for GSDMD antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GSDMD antibody
Flow Cytometry (FC) protocol for GSDMD antibody
Publications
Application:
Species:
Post-burns persistent inflammation leads to kidney PANoptosis with Caspases pathway activation
Published: 2024-12-27
Journal: Heliyon
Application: WB,IHC
Impact Factor: 3.40
Species: Mouse
Diabetes Mellitus Inhibits Hair Follicle Regeneration by Inducing Macrophage Reprogramming-Mediated Pyroptosis
Published: 2024-09-30
Journal: Journal of Inflammation Research
Application: IHC
Impact Factor: 4.20
Species: Mouse
PMID: 39372592
KIF23 promotes cervical cancer progression via inhibiting NLRP3-mediated pyroptosis
Published: 2024-05-23
Journal: FASEB JOURNAL
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 38780518
Hesperidin Protects Against High-Fat Diet-Induced Lipotoxicity in Rats by Inhibiting Pyroptosis
Published: 2024-01-30
Journal: JOURNAL OF MEDICINAL FOOD
Application: IHC
Impact Factor: 1.70
Species: Rat
PMID: 38294790
Polystyrene microplastics-induced cardiotoxicity in chickens via the ROS-driven NF-κB-NLRP3-GSDMD and AMPK-PGC-1α axes
Published: 2022-06-14
Journal: SCIENCE OF THE TOTAL ENVIRONMENT
Application: WB, IHC
Impact Factor: 10.75
Species: Chicken
PMID: 35714743
Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis
Published: 2021-11-16
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: IHC
Impact Factor: 6.29
Species: Mouse
PMID: 34798361
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Product Promise
We promise Anti-GSDMD Antibody Picoband® (monoclonal, 6D11) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M02842
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026