Western blot analysis of GSK3 Alpha/Beta (Phospho-Y216+Y279) using anti-GSK3 Alpha/Beta (Phospho-Y216+Y279) antibody (P03152-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 Alpha/Beta (Phospho-Y216+Y279) antigen affinity purified monoclonal antibody (P03152-1) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSK3 Alpha/Beta (Phospho-Y216+Y279) at approximately 47,51 kDa. The expected band size for GSK3 Alpha/Beta (Phospho-Y216+Y279) is at 47,51 kDa.