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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-GSK3 alpha/GSK3A Antibody Picoband™
Boster Bio Anti-GSK3 alpha/GSK3A Antibody Picoband™ catalog # A03152-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GSK3 alpha/GSK3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03152-2)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of human GSK3 alpha (QEVAYTDIKVIGNGSFGVVYQARLAETRELVAIKKVLQDKR).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A03152-2 is reactive to GSK3A in Human, Mouse, Rat
A03152-2 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GSK3 alpha using anti-GSK3 alpha antibody (A03152-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: rat testis tissue lysate,
Lane 3: mouse testis tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 alpha antigen affinity purified polyclonal antibody (Catalog # A03152-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 alpha at approximately 51KD. The expected band size for GSK3 alpha is at 51KD.
Gene/Protein Information For GSK3A (Source: Uniprot.Org, NCBI)
Glycogen synthase kinase-3 alpha
protein kinase superfamily
DKFZp686D0638; EC 2.7.11; EC 184.108.40.206; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK3 alpha; GSK-3 alpha; GSK3A GSK3A glycogen synthase kinase 3 alpha glycogen synthase kinase-3 alpha|GSK-3 alpha|serine/threonine-protein kinase GSK3A*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on GSK3A, check out the GSK3A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GSK3A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A03152-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mcl-1 stabilization confers resistance to taxol in human gastric cancer
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