Anti-GTPBP9/OLA1 Antibody Picoband™

Product Name

Anti-GTPBP9/OLA1 Antibody Picoband™

View all GTPBP9 Antibodies

SKU/Catalog Number

A07162

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-GTPBP9/OLA1 Antibody Picoband™ catalog # A07162. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GTPBP9/OLA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07162)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GTPBP9/OLA1 recombinant protein (Position: D8-D379).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07162 is reactive to OLA1 in Human, Mouse, Rat

Applications

A07162 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

44.744kDa

Background of GTPBP9

Obg-like ATPase 1 is an enzyme that in humans is encoded by the OLA1 gene. This gene encodes a member of the GTPase protein family. The encoded protein interacts with breast cancer-associated gene 1 (BRCA1) and BRCA1-associated RING domain protein (BARD1), and is involved in centrosome regulation. Overexpression of this gene has been observed in multiple types of cancer and may be associated with poor survival. Pseudogenes of this gene have been defined on chromosomes 17 and 22.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of GTPBP9/OLA1 using anti-GTPBP9/OLA1 antibody (A07162).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTPBP9/OLA1 antigen affinity purified polyclonal antibody (Catalog # A07162) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GTPBP9/OLA1 at approximately 45 kDa. The expected band size for GTPBP9/OLA1 is at 45 kDa.

Click image to see more details

Figure 2. IF analysis of GTPBP9/OLA1 using anti-GTPBP9/OLA1 antibody (A07162).
GTPBP9/OLA1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GTPBP9/OLA1 Antibody (A07162) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Click image to see more details

Figure 3. Flow Cytometry analysis of Raji cells using anti-GTPBP9/OLA1 antibody (A07162).
Overlay histogram showing Raji cells stained with A07162 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GTPBP9/OLA1 Antibody (A07162, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For OLA1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp313H1942; DNA damage-regulated overexpressed in cancer 45 protein; DOC45; EC 3.6.3; EC 3.6.3.-; GBP45; GTP-binding protein 9 (putative); GTP-binding protein 9; GTP-binding protein PTD004; GTPBP9; homologous yeast-44.2 protein; Obg-like ATPase 1; PTD004 OLA1 DOC45, GBP45, GTBP9, GTPBP9, PTD004 Obg like ATPase 1 obg-like ATPase 1|DNA damage-regulated overexpressed in cancer 45 protein|GTP-binding protein 9 (putative)|GTP-binding protein PTD004|homologous yeast-44.2 protein

For more info on OLA1, check out the OLA1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for OLA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the OLA1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis