Anti-HA Tag Plus Antibody (monoclonal, 1A4)

Product Name

Anti-HA Tag Plus Antibody (monoclonal, 1A4)

View all Tmem177 Antibodies

SKU/Catalog Number

M30974

Size

100μl/vial

Form

Liquid

Description

Boster Bio Anti-HA Tag Plus Antibody (monoclonal, 1A4) catalog # M30974. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HA Tag Plus Antibody (monoclonal, 1A4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30974)

Host

Mouse

Contents

0.5mg/ml in PBS, pH7.4 and 50% glycerol.

Clonality

Monoclonal

Clone Number

1A4

Isotype

IgG1

Immunogen

This antibody is produced by immunizing animals with a synthetic HA tag peptide corresponding to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M30974 is reactive to Tmem177 in All species

Applications

M30974 is guaranteed for Dot blot, ELISA, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

34.067kDa

Background of Tmem177

The HA Tag is derived from the human influenza hemagglutinin corresponding to amino acids 98-106. It has been extensively used as a general epitope Tag in expression vectors. This Tag facilitates the detection, isolation, and purification of the proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

1:500-1:5000

Validation Images & Assay Conditions

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Western blot analysis of different concentration of Anti-HA(1A4) Monoclonal Antibody
Antigen: Extracts from E.coli expressing HA-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, 1PBS, 4°C, overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, 1TBS, 0.1% Tween-20, RT, 30mins

Gene/Protein Information For Tmem177 (Source: Uniprot.org, NCBI)

Alternative Names

MGC10993; transmembrane protein 177

For more info on Tmem177, check out the Tmem177 Infographic

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In this infographic, you will see the following information for Tmem177: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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