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Product Info Summary
| SKU: | M04757 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-HACE1 Antibody
SKU/Catalog Number
M04757
Size
50 µl
Description
Boster Bio Anti-HACE1 Antibody (Catalog # M04757). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HACE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04757)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1854CT757.66.47
Isotype
IgG1,k
Immunogen
This HACE1 antibody is generated from a mouse immunized with a recombinant protein from the human region of human HACE1.
Reactive Species
M04757 is reactive to HACE1 in Human
Calculated molecular weight
102.3 kDa
Background of HACE1
E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04757 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-HACE1 Antibody at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: 293T/17 whole cell lysate
e
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 102 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-HACE1 Antibody (M04757)
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