Anti-HACE1 Antibody

Product Name

Anti-HACE1 Antibody

View all HACE1 Antibodies

SKU/Catalog Number

M04757

Size

50 µl

Description

Boster Bio Anti-HACE1 Antibody (Catalog # M04757). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-HACE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04757)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1854CT757.66.47

Isotype

IgG1,k

Immunogen

This HACE1 antibody is generated from a mouse immunized with a recombinant protein from the human region of human HACE1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04757 is reactive to HACE1 in Human

Applications

M04757 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

102.342kDa

Background of HACE1

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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All lanes : Anti-HACE1 Antibody at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: 293T/17 whole cell lysate
e
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 102 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For HACE1 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase HACE1; EC 6.3.2; HACE; HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; KIAA1320EC 6.3.2.- HACE1 SPPRS HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase HACE1|HECT-type E3 ubiquitin transferase HACE1

For more info on HACE1, check out the HACE1 Infographic

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In this infographic, you will see the following information for HACE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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