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Product Info Summary
| SKU: | M03375 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-HAS2 Antibody (Center)
SKU/Catalog Number
M03375
Size
50 µl
Description
Boster Bio Anti-HAS2 Antibody (Center) (Catalog # M03375). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HAS2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03375)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2105CT879.18.75
Isotype
IgG1,κ
Immunogen
This HAS2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 133-166 amino acids from the Central region of human HAS2.
Reactive Species
M03375 is reactive to HAS2 in Human
Calculated molecular weight
63.6 kDa
Background of HAS2
Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03375 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
Validation Images & Assay Conditions
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Anti-HAS2 Antibody (Center) at 1:8000 dilution + SW620 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 64 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-HAS2 Antibody (Center) (M03375)
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