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Product Info Summary
| SKU: | A08093-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB, IHC-P-Leica |
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Product info
Product Name
Anti-HBE1 Antibody(Center)
SKU/Catalog Number
A08093-1
Size
80 µl
Description
Boster Bio Anti-HBE1 Antibody(Center) (Catalog # A08093-1). Tested in WB, Flow Cytometry, IHC-P-Leica application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HBE1 Antibody(Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08093-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the Central region of human HBE1.
Reactive Species
A08093-1 is reactive to HBE1 in Human
Calculated molecular weight
16.2 kDa
Background of HBE1
The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3'
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08093-1 is guaranteed for Flow Cytometry, WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
IHC-P-Leica: 1:500
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-HBE1 Antibody (Center) at 1:1000 dilution
Lane 1: human placenta lysate
Lane 2: human kidney lysate
Lane 3: human spleen lysate
Lane 4: human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 16 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-HBE1 Antibody(Center) at 1:1000 dilution + NCCIT whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 16 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-HBE1 Antibody (Center) at 1:8000 dilution
Lane 1: human placenta lysate
Lane 2: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 16 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using A08093-1 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Overlay histogram showing K562 cells stained with A08093-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A08093-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-HBE1 Antibody(Center) (A08093-1)
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