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Product Info Summary
| SKU: | A01990 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-HBG2 Antibody (C-term)
SKU/Catalog Number
A01990
Size
80 µl
Description
Boster Bio Anti-HBG2 Antibody (C-term) (Catalog # A01990). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HBG2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01990)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HBG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-133 amino acids from the C-terminal region of human HBG2.
Reactive Species
A01990 is reactive to HBG2 in Human, Mouse
Calculated molecular weight
16.1 kDa
Background of HBG2
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01990 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from human brain tissue lysate, using HBG2 Antibody (C-term) . A01990 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody (C-term) (Cat#A01990). A01990 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody (C-term) (Cat#A01990). A01990 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term) (green, Cat#A01990) compared to an isotype control of rabbit IgG (blue). A01990 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-HBG2 Antibody (C-term) (A01990)
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