Anti-HDAC-4 (internal) Antibody

Product Name

Anti-HDAC-4 (internal) Antibody

View all HDAC4 Antibodies

SKU/Catalog Number

A00971-2

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HDAC-4 (internal) Antibody (Catalog # A00971-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Primate.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.

Cite This Product

Anti-HDAC-4 (internal) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00971-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00971-2 is reactive to HDAC4 in Human, Mouse, Rat, Primate

Applications

A00971-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

119.04kDa

Background of HDAC4

HDAC4 is a 140 kD class II histone deacetylase similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus. Anti-HDAC4 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1 mg/mL
IF Microscopy: 1:100-1:500
WB: 1 mg/mL

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HDAC4 using anti-HDAC4 antibody (A00971-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC4 antigen affinity purified polyclonal antibody (Catalog # A00971-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HDAC4.

Gene/Protein Information For HDAC4 (Source: Uniprot.org, NCBI)

Alternative Names

HA6116; HD4EC 3.5.1.98; HDAC4; HDAC-A; HDACABDMR; Histone Deacetylase 4; histone deacetylase A; KIAA0288AHO3 HDAC4 AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA histone deacetylase 4 histone deacetylase 4|histone deacetylase A

For more info on HDAC4, check out the HDAC4 Infographic

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In this infographic, you will see the following information for HDAC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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