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Product Info Summary
| SKU: | M00971 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00971
BM4750 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal Antibody catalog # M00971. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00971)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HGI-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC4
Reactive Species
M00971 is reactive to HDAC4 in Human
Observed Molecular Weight
140 kDa
Calculated molecular weight
119.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00971 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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Western blot analysis of HDAC4 expression in Hela lysate.
Specific Publications For Anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal Antibody (M00971)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for brain using anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal antibody M00971. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
Would anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal antibody M00971 work for WB with brain?
N. Collins
Verified customer
Asked: 2019-05-29
Answer
According to the expression profile of brain, HDAC4 is highly expressed in brain. So, it is likely that anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal antibody M00971 will work for WB with brain.
Boster Scientific Support
Answered: 2019-05-29
Question
Do you have a BSA free version of anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal antibody M00971 available?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal antibody M00971 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-25
Question
I was wanting to use your anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
M. Jones
Verified customer
Asked: 2017-01-09
Answer
As indicated on the product datasheet, M00971 anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-09
