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Product Info Summary
| SKU: | A12287-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HDDC3 Antibody Picoband®
SKU/Catalog Number
A12287-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HDDC3 Antibody Picoband® catalog # A12287-1. Tested in WB,ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HDDC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12287-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human HDDC3 recombinant protein (Position: K25-I179). Human HDDC3 shares 95.5% amino acid (aa) sequence identity with mouse HDDC3.
Reactive Species
A12287-1 is reactive to HDDC3 in Human, Mouse
Observed Molecular Weight
22 kDa
Calculated molecular weight
20.3 kDa
Background of HDDC3
HDDC3, also known as Histone Deacetylase Domain Containing 3, is a gene that encodes a protein with a potential histone deacetylase activity. Proteins in this family are involved in the modification of histones, which can affect gene expression and cellular processes. Although the precise functions of HDDC3 are not fully understood, it is likely to play a role in regulating gene expression and cellular differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12287-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse heart tissue
Validation Images & Assay Conditions
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Western blot analysis of HDDC3 using anti-HDDC3 antibody (A12287-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDDC3 antigen affinity purified polyclonal antibody (Catalog # A12287-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDDC3 at approximately 22 kDa. The expected band size for HDDC3 is at 20 kDa.
Specific Publications For Anti-HDDC3 Antibody Picoband® (A12287-1)
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