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Product Info Summary
| SKU: | A17234-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HDHD3 Antibody Picoband®
SKU/Catalog Number
A17234-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HDHD3 Antibody Picoband® catalog # A17234-1. Tested in WB, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HDHD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17234-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human HDHD3 recombinant protein (Position: M1-E245). Human HDHD3 shares 77.6% amino acid (aa) sequence identity with mouse HDHD3.
Reactive Species
A17234-1 is reactive to HDHD3 in Human, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.0 kDa
Background of HDHD3
Predicted to enable hydrolase activity. Located in nucleolus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17234-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of HDHD3 using anti-HDHD3 antibody (A17234-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat small intestine tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDHD3 antigen affinity purified polyclonal antibody (A17234-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HDHD3 at approximately 28 kDa. The expected band size for HDHD3 is at 28 kDa.
Specific Publications For Anti-HDHD3 Antibody Picoband® (A17234-1)
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