Product Info Summary
SKU: | PA1597 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HDJ2/DNAJA1 Antibody
SKU/Catalog Number
PA1597
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HDJ2/DNAJA1 Antibody catalog # PA1597. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDJ2/DNAJA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1597)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HDJ2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1597 is reactive to DNAJA1 in Human, Mouse, Rat
Applications
PA1597 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.868kDa
Background of HDJ2
DNAJA1 (DnaJ homolog subfamily A member 1), also called DJA1, HSDJ, HSPF4, is a protein that in humans is encoded by the DNAJA1 gene. The deduced 397-amino acid DNAJA1 protein is 32% identical to E. coli DnaJ, with the highest identity in the N-terminal region. Among the known DNAJ homologs in S. cerevisiae, DNAJA1 is most identical to YDJ1, which may be involved in the transport of certain proteins into the mitochondria and endoplasmic reticulum. Dja1 is expressed in mouse testis and prostate by immunohistochemical analysis and Western blot analysis. Transplantation experiments with fluorescence-tagged spermatogonia into Dja1 -/- mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-HDJ2 antibody, PA1597, Western blotting
All lanes: Anti HDJ2 (PA1597) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: A431 Whole Cell Lysate at 40ug
Lane 9: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
Click image to see more details
Figure 2. IF analysis of DNAJA1 using anti-DNAJA1 antibody (PA1597).
DNAJA1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-DNAJA1 Antibody (PA1597) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For DNAJA1 (Source: Uniprot.org, NCBI)
Gene Name
DNAJA1
Full Name
DnaJ homolog subfamily A member 1
Weight
44.868kDa
Alternative Names
dj-2; DjA1; DnaJ (Hsp40) homolog, subfamily A, member 1; dnaJ homolog subfamily A member 1; DnaJ protein homolog 2; DNAJ2; HDJ2; hDJ-2; Heat shock 40 kDa protein 4; Heat shock protein J2; heat shock protein, DNAJ-like 2; HSDJ; HSJ2; HSJ-2; HSPF4hdj-2; Human DnaJ protein 2; NEDD7; neural precursor cell expressed, developmentally down-regulated 7 DNAJA1 DJ-2, DjA1, HDJ2, HSDJ, HSJ-2, HSJ2, HSPF4, NEDD7, hDJ-2 DnaJ heat shock protein family (Hsp40) member A1 dnaJ homolog subfamily A member 1|DnaJ (Hsp40) homolog, subfamily A, member 1|dnaJ protein homolog 2|heat shock 40 kDa protein 4|heat shock protein J2|heat shock protein, DNAJ-like 2|human DnaJ protein 2|neural precursor cell expressed, developmentally down-regulated 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNAJA1, check out the DNAJA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNAJA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDJ2/DNAJA1 Antibody (PA1597)
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No publications found for PA1597
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-HDJ2/DNAJA1 Antibody
Question
We are currently using anti-HDJ2/DNAJA1 antibody PA1597 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
The anti-HDJ2/DNAJA1 antibody (PA1597) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-17