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Product Info Summary
| SKU: | M01731 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-HEC1 NDC80 Monoclonal Antibody
SKU/Catalog Number
M01731
BM5230 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HEC1 NDC80 Monoclonal Antibody catalog # M01731. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HEC1 NDC80 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01731)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACBI-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Reactive Species
M01731 is reactive to NDC80 in Human, Mouse, Rat
Observed Molecular Weight
74 kDa
Calculated molecular weight
73.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01731 is guaranteed for IP, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
IP 1:20
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of HEC1 expression in Jurkat cell lysate.
Specific Publications For Anti-HEC1 NDC80 Monoclonal Antibody (M01731)
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Customer Q&As
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4 Customer Q&As for Anti-HEC1 NDC80 Monoclonal Antibody
Question
Is this M01731 anti-HEC1 Monoclonal antibody reactive to the isotypes of NDC80?
M. Johnson
Verified customer
Asked: 2019-03-25
Answer
The immunogen of M01731 anti-HEC1 Monoclonal antibody is A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-25
Question
Will anti-HEC1 Monoclonal antibody M01731 work on primate IP with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
Our lab technicians have not tested anti-HEC1 Monoclonal antibody M01731 on primate. You can run a BLAST between primate and the immunogen sequence of anti-HEC1 Monoclonal antibody M01731 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-19
Question
I was wanting to use to test anti-HEC1 Monoclonal antibody M01731 on human cervix carcinoma for research purposes, then I may be interested in using anti-HEC1 Monoclonal antibody M01731 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Jones
Verified customer
Asked: 2014-08-29
Answer
The products we sell, including anti-HEC1 Monoclonal antibody M01731, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-29
Question
I see that the anti-HEC1 Monoclonal antibody M01731 works with IP, what is the protocol used to produce the result images on the product page?
M. Johnson
Verified customer
Asked: 2014-03-11
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-11
