Anti-HECTD3 Antibody Picoband®

Hectd3 antibody

Boster Bio Anti-HECTD3 Antibody Picoband® catalog # A11560. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A11560
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, IHC-F, ICC, WB

Product Name

Anti-HECTD3 Antibody Picoband®

View all Hectd3 Antibodies

SKU/Catalog Number

A11560

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HECTD3 Antibody Picoband® catalog # A11560. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HECTD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11560)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HECTD3, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A11560 is reactive to HECTD3 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

97 kDa

Calculated molecular weight

97.347kDa

Background of Hectd3

The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. This gene is mapped to 1p34.1. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A11560 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, Human

Positive Control

WB: human Caco-2 whole cell, rat brain tissue, mouse brain tissue
IHC: human lung cancer tissue, human lung cancer tissue, mouse gaster tissue
FCM: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For HECTD3 (Source: Uniprot.org, NCBI)

Gene Name

HECTD3

Full Name

E3 ubiquitin-protein ligase HECTD3

Weight

97.347kDa

Alternative Names

E3 ubiquitin-protein ligase HECTD3; HECT domain-containing protein 3; HECT-type E3 ubiquitin transferase HECTD3; HECTD3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HECTD3, check out the HECTD3 Infographic

HECTD3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HECTD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A11560

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-HECTD3 Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-HECTD3 Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Anti-HECTD3 Antibody Picoband®

Question

We are currently using anti-HECTD3 antibody A11560 for rat tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-01-16

Answer

The anti-HECTD3 antibody (A11560) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-16

Question

Our lab want to know about to test anti-HECTD3 antibody A11560 on human erythroleukemia for research purposes, then I may be interested in using anti-HECTD3 antibody A11560 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

The products we sell, including anti-HECTD3 antibody A11560, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-30

Question

I have attached the WB image, lot number and protocol we used for erythroleukemia using anti-HECTD3 antibody A11560. Please let me know if you require anything else.

L. Anderson

Verified customer

Asked: 2015-06-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-06-17

Order DetailsPrice
A11560

100μg

$370
A11560-10ug

10μg sample (liquid)

$99
A11560-Biotin

100 μg Biotin conjugated

$570
A11560-Cy3

100 μg Cy3 conjugated

$570
A11560-Dylight488

100 μg Dylight488 conjugated

$570
A11560-Dylight550

100 μg Dylight550 conjugated

$570
A11560-Dylight594

100 μg Dylight594 conjugated

$570
A11560-FITC

100 μg FITC conjugated

$570
A11560-HRP

100 μg HRP conjugated

$570
A11560-APC

100 μg APC conjugated

$670
A11560-PE

100 μg PE conjugated

$670
A11560-iFluor647

100 μg iFluor647 conjugated

$670
A11560-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A11560
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.