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Product Info Summary
| SKU: | A08422 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HEMGN Antibody (C-term)
SKU/Catalog Number
A08422
Size
80 µl
Description
Boster Bio Anti-HEMGN Antibody (C-term) (Catalog # A08422). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HEMGN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08422)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HEMGN antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 355-389 amino acids from the C-terminal region of human HEMGN.
Reactive Species
A08422 is reactive to HEMGN in Human
Calculated molecular weight
55.3 kDa
Background of HEMGN
Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08422 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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Western blot analysis of lysate from Jurkat cell line, using HEMGN Antibody (C-term). A08422 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Anti-HEMGN Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 55 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-HEMGN Antibody (C-term) (A08422)
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