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Product Info Summary
| SKU: | A08422 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HEMGN Antibody (C-term)
SKU/Catalog Number
A08422
Size
80 µl
Description
Boster Bio Anti-HEMGN Antibody (C-term) (Catalog # A08422). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HEMGN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08422)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HEMGN antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 355-389 amino acids from the C-terminal region of human HEMGN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08422 is reactive to HEMGN in Human
Reconstitution
Calculated molecular weight
55341 Da
Background of Hemogen
Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08422 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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Western blot analysis of lysate from Jurkat cell line, using HEMGN Antibody (C-term). A08422 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Anti-HEMGN Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 55 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For HEMGN (Source: Uniprot.org, NCBI)
Gene Name
HEMGN
Full Name
Hemogen
Weight
55341 Da
Alternative Names
Hemogen, Erythroid differentiation-associated gene protein, EDAG-1, Hemopoietic gene protein, Negative differentiation regulator protein, HEMGN, EDAG, NDR HEMGN CT155, EDAG, EDAG-1, NDR hemogen hemogen|erythroid differentiation-associated gene protein|hemopoietic gene protein|negative differentiation regulator protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HEMGN, check out the HEMGN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HEMGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HEMGN Antibody (C-term) (A08422)
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