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Product Info Summary
| SKU: | A30472 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HEN1/2 NHLH1 Antibody
SKU/Catalog Number
A30472
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HEN1/2 NHLH1 Antibody (Catalog# A30472). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HEN1/2 NHLH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30472)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HEN1/2. Immunogen sequence location: 41-90
Reactive Species
A30472 is reactive to NHLH1 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
14.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30472 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using HEN1/2 Polyclonal Antibody diluted at 1:1000.
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Western blot analysis of lysates from Jurkat cells, using HEN1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-HEN1/2 NHLH1 Antibody (A30472)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-HEN1/2 NHLH1 Antibody
Question
Is there a BSA free version of anti-HEN1/2 antibody A30472 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HEN1/2 antibody A30472 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
Will anti-HEN1/2 antibody A30472 work for WB with eye?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
According to the expression profile of eye, NHLH1 is highly expressed in eye. So, it is likely that anti-HEN1/2 antibody A30472 will work for WB with eye.
Boster Scientific Support
Answered: 2019-09-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-HEN1/2 antibody A30472. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-09
Question
We are currently using anti-HEN1/2 antibody A30472 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The anti-HEN1/2 antibody (A30472) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-17
Question
I would like to test anti-HEN1/2 antibody A30472 on human eye for research purposes, then I may be interested in using anti-HEN1/2 antibody A30472 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-11
Answer
The products we sell, including anti-HEN1/2 antibody A30472, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-11
