Anti-HEN1/2 NHLH1 Antibody

NHLH1 antibody

Boster Bio Anti-HEN1/2 NHLH1 Antibody (Catalog# A30472). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A30472
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

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Product Name

Anti-HEN1/2 NHLH1 Antibody

View all NHLH1 Antibodies

SKU/Catalog Number

A30472

Size

100ul

Form

Liquid

Description

Boster Bio Anti-HEN1/2 NHLH1 Antibody (Catalog# A30472). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HEN1/2 NHLH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30472)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human HEN1/2. Immunogen sequence location: 41-90

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30472 is reactive to NHLH1 in Human, Mouse

Applications

A30472 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

14.618kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For NHLH1 (Source: Uniprot.org, NCBI)

Gene Name

NHLH1

Full Name

Helix-loop-helix protein 1

Weight

14.618kDa

Alternative Names

BHLHA35; bHLHa35HEN-1; helix-loop-helix protein 1; HEN1NSCL-1; nescient helix loop helix 1Class A basic helix-loop-helix protein 35; NSCL; NSCL1 NHLH1 HEN1, NSCL, NSCL1, bHLHa35 nescient helix-loop-helix 1 helix-loop-helix protein 1|HEN-1|NSCL-1|class A basic helix-loop-helix protein 35

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NHLH1, check out the NHLH1 Infographic

NHLH1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NHLH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-HEN1/2 NHLH1 Antibody

Question

Is there a BSA free version of anti-HEN1/2 antibody A30472 available?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HEN1/2 antibody A30472 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-20

Question

Will anti-HEN1/2 antibody A30472 work for WB with eye?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

According to the expression profile of eye, NHLH1 is highly expressed in eye. So, it is likely that anti-HEN1/2 antibody A30472 will work for WB with eye.

Boster Scientific Support

Answered: 2019-09-18

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-HEN1/2 antibody A30472. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-09

Question

We are currently using anti-HEN1/2 antibody A30472 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

The anti-HEN1/2 antibody (A30472) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-17

Question

I would like to test anti-HEN1/2 antibody A30472 on human eye for research purposes, then I may be interested in using anti-HEN1/2 antibody A30472 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-05-11

Answer

The products we sell, including anti-HEN1/2 antibody A30472, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-11

Order DetailsPrice
A30472

100 μL

$335

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