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HEPH antibody (A05316-1) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of EL-4 cells using anti-Hephaestin/HEPH antibody (A05316-1).
Overlay histogram showing EL-4 cells stained with A05316-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Hephaestin/HEPH Antibody (A05316-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing EL-4 cells stained with A05316-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Hephaestin/HEPH Antibody (A05316-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Hephaestin/HEPH Antibody Picoband®
HEPH antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
HEPH antibody was positively detected by WB in:
rat C6 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HEPH antibody was positively detected by IHC in:
human small intestine tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HEPH antibody was positively detected by FC in:
EL-4 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Hephaestin/HEPH Antibody Picoband®
View all HEPH Antibodies
View all HEPH Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Hephaestin/HEPH recombinant protein (Position: E348-Q1157). Mouse Hephaestin/HEPHshares 85.6% and 96.2% amino acid (aa) sequence identity with human and rat Hephaestin/HEPH, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
129.7 kDa
Background of HEPH
Enables ferroxidase activity. Involved in intestinal iron absorption; multicellular organismal-level iron ion homeostasis; and positive regulation of iron export across plasma membrane. Acts upstream of or within erythrocyte differentiation. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in basolateral plasma membrane. Orthologous to human HEPH (hephaestin).
Cite This Product
Anti-Hephaestin/HEPH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05316-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
96%
High Homology
Human
86%
Moderate Homology
Monkey
86%
Moderate Homology
Rabbit
85%
Moderate Homology
Sheep
85%
Moderate Homology
Dog
85%
Moderate Homology
Cow
85%
Moderate Homology
Zebrafish
45%
Low homology
Drosophila
1%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HEPH antibody
Immunohistochemistry (IHC) protocol for HEPH antibody
Flow Cytometry (FC) protocol for HEPH antibody
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Product Promise
We promise Anti-Hephaestin/HEPH Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05316-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 29, 2026