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Product Info Summary
| SKU: | A09854-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HEXIM2 Antibody Picoband®
SKU/Catalog Number
A09854-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HEXIM2 Antibody Picoband® catalog # A09854-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HEXIM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09854-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human HEXIM2 recombinant protein (Position: Q243-Q268). Human HEXIM2 shares 88% amino acid (aa) sequence identity with mouse HEXIM2.
Reactive Species
A09854-2 is reactive to HEXIM2 in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
32.4 kDa
Background of HEXIM2
Predicted to enable 7SK snRNA binding activity; cyclin-dependent protein serine/threonine kinase inhibitor activity; and identical protein binding activity. Predicted to be involved in negative regulation of G2/M transition of mitotic cell cycle and negative regulation of transcription by RNA polymerase II. Predicted to be located in cytosol and nuclear speck. Predicted to be active in cytoplasm and nucleoplasm. Is expressed in heart and skeletal muscle. Orthologous to human HEXIM2 (HEXIM P-TEFb complex subunit 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09854-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of HEXIM2 using anti-HEXIM2 antibody (A09854-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human COL0320 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXIM2 antigen affinity purified polyclonal antibody (A09854-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HEXIM2 at approximately 38 kDa. The expected band size for HEXIM2 is at 35 kDa.
Specific Publications For Anti-HEXIM2 Antibody Picoband® (A09854-2)
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