Anti-Hexokinase-3 HK3 Antibody

Hexokinase Type III antibody

Boster Bio Anti-Hexokinase-3 HK3 Antibody (Catalog # A05145). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A05145
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Hexokinase-3 HK3 Antibody

View all Hexokinase Type III Antibodies

SKU/Catalog Number

A05145

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Hexokinase-3 HK3 Antibody (Catalog # A05145). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hexokinase-3 HK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05145)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human Hexokinase-3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05145 is reactive to HK3 in Human

Applications

A05145 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

99.025kDa

Background of Hexokinase Type III

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

Furuta H., Genomics 36:206-209(1996).
Palma F., Mol. Cell. Biochem. 155:23-29(1996).
Sjoeblom T., Science 314:268-274(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For HK3 (Source: Uniprot.org, NCBI)

Gene Name

HK3

Full Name

Hexokinase-3

Weight

99.025kDa

Superfamily

hexokinase family

Alternative Names

ATP:D-hexose 6-phosphotransferase; EC 2.7.1; EC 2.7.1.1; hexokinase 3 (white cell); Hexokinase type III; hexokinase-3; HK III; HKIII; HXK3 HK3 HKIII, HXK3 hexokinase 3 hexokinase-3|ATP:D-hexose 6-phosphotransferase|HK III|hexokinase 3 (white cell)|hexokinase type III|hexokinase-C

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HK3, check out the HK3 Infographic

HK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Hexokinase-3 HK3 Antibody

Question

Please see the WB image, lot number and protocol we used for blood using anti-Hexokinase-3 antibody A05145. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-21

Question

Does A05145 anti-Hexokinase-3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Wu

Verified customer

Asked: 2019-01-09

Answer

As indicated on the product datasheet, A05145 anti-Hexokinase-3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-09

Question

I was wanting to use your anti-Hexokinase-3 antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?

Verified Customer

Verified customer

Asked: 2018-08-01

Answer

You can see on the product datasheet, A05145 anti-Hexokinase-3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-01

Question

Is there a BSA free version of anti-Hexokinase-3 antibody A05145 available?

V. Krishna

Verified customer

Asked: 2017-06-14

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hexokinase-3 antibody A05145 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-14

Question

Is this A05145 anti-Hexokinase-3 antibody reactive to the isotypes of HK3?

B. Carter

Verified customer

Asked: 2016-08-31

Answer

The immunogen of A05145 anti-Hexokinase-3 antibody is Synthesized peptide derived from internal of human Hexokinase-3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-08-31

Question

We are currently using anti-Hexokinase-3 antibody A05145 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

G. Krishna

Verified customer

Asked: 2014-03-17

Answer

The anti-Hexokinase-3 antibody (A05145) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-03-17

Order DetailsPrice
A05145-100uL

100uL

$360

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