Anti-Hfe Antibody Picoband™

HFE antibody

Boster Bio Anti-Hfe Antibody Picoband™ catalog # A00506-5. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00506-5
Size: 100 µg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, WB

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Product Name

Anti-Hfe Antibody Picoband™

View all HFE Antibodies

SKU/Catalog Number

A00506-5

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hfe Antibody Picoband™ catalog # A00506-5. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Hfe Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00506-5)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Hfe recombinant protein (Position: N112-H227).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00506-5 is reactive to HFE in Mouse, Rat

Applications

A00506-5 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

40-58 kDa

Calculated molecular weight

40.108kDa

Background of HFE

Human homeostatic iron regulator protein, also known as the HFE protein (High FE2+), is a protein which in humans is encoded by the HFE gene. Enables transferrin receptor binding activity. Involved in positive regulation of gene expression. Acts upstream of or within hormone biosynthetic process and multicellular organismal iron ion homeostasis. Part of HFE-transferrin receptor complex. Is expressed in brain; choroid invagination; diencephalon roof plate; medulla oblongata part of 4th ventricle choroid plexus; and metencephalon part of 4th ventricle choroid plexus. Used to study hemochromatosis type 1. Human ortholog(s) of this gene implicated in several diseases, including acute porphyria (multiple); arthritis (multiple); bone marrow cancer (multiple); hemochromatosis (multiple); and liver disease (multiple). Orthologous to human HFE (homeostatic iron regulator).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Rat
Flow Cytometry, 1-3 µg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For HFE (Source: Uniprot.org, NCBI)

Gene Name

HFE

Full Name

Hereditary hemochromatosis protein

Weight

40.108kDa

Superfamily

MHC class I family

Alternative Names

dJ221C16.10.1; hemochromatosis; hereditary hemochromatosis protein HLA-H; hereditary hemochromatosis protein; HH; high Fe; HLAH; HLA-HHFE1; MGC103790; MHC class I-like protein HFE; MVCD7 HFE HFE1, HH, HLA-H, MVCD7, TFQTL2 homeostatic iron regulator hereditary hemochromatosis protein|MHC class I-like protein HFE|hereditary hemochromatosis protein HLA-H|high Fe

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HFE, check out the HFE Infographic

HFE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HFE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00506-5

100μg

$370
A00506-5-10ug

10μg sample (liquid)

$99
A00506-5-Biotin

100 µg Biotin conjugated

$570
A00506-5-Cy3

100 µg Cy3 conjugated

$570
A00506-5-Dylight488

100 µg Dylight488 conjugated

$570
A00506-5-Dylight550

100 µg Dylight550 conjugated

$570
A00506-5-Dylight594

100 µg Dylight594 conjugated

$570
A00506-5-FITC

100 µg FITC conjugated

$570
A00506-5-HRP

100 µg HRP conjugated

$570
A00506-5-APC

100 µg APC conjugated

$670
A00506-5-PE

100 µg PE conjugated

$670
A00506-5-carrier-free

Carrier Free

$370

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A00506-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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