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Product Info Summary
| SKU: | A09351 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HGFA HGFAC Antibody
SKU/Catalog Number
A09351
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HGFA HGFAC Antibody catalog # A09351. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HGFA HGFAC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09351)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human HGFA.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09351 is reactive to HGFAC in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
70.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09351 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from K562, Jurkat cells, using HGFA Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-HGFA HGFAC Antibody (A09351)
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Customer Q&As
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5 Customer Q&As for Anti-HGFA HGFAC Antibody
Question
Does anti-HGFA antibody A09351 work for WB with liver serum?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
According to the expression profile of liver serum, HGFAC is highly expressed in liver serum. So, it is likely that anti-HGFA antibody A09351 will work for WB with liver serum.
Boster Scientific Support
Answered: 2020-04-22
Question
Here is the WB image, lot number and protocol we used for liver serum using anti-HGFA antibody A09351. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
We are currently using anti-HGFA antibody A09351 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-HGFA antibody (A09351) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
Our lab want to know about to test anti-HGFA antibody A09351 on human liver serum for research purposes, then I may be interested in using anti-HGFA antibody A09351 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
The products we sell, including anti-HGFA antibody A09351, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-21
Question
Can you help my question with product A09351, anti-HGFA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Dhar
Verified customer
Asked: 2016-06-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09351 anti-HGFA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-01
