Anti-HIF3/HIF3A Antibody Picoband™

Product Name

Anti-HIF3/HIF3A Antibody Picoband™

View all HIF-3 alpha Antibodies

SKU/Catalog Number

A03805-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HIF3/HIF3A Antibody Picoband™ catalog # A03805-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HIF3/HIF3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03805-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HIF3/HIF3A recombinant protein (Position: Q29-Q480).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03805-3 is reactive to Hif3a in Human, Mouse, Rat

Applications

A03805-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

73.044kDa

Background of HIF-3 alpha

Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.

Click image to see more details

Figure 2. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.

Click image to see more details

Figure 3. Flow Cytometry analysis of U87 cells using anti-HIF3A antibody (A03805-3).
Overlay histogram showing U87 cells stained with A03805-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF3A Antibody (A03805-3, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Hif3a (Source: Uniprot.org, NCBI)

Alternative Names

Basic-helix-loop-helix-PAS protein MOP7; BHLHE17; bHLHe17HIF-3A2; Class E basic helix-loop-helix protein 17; HIF-3A4; HIF-3-alpha; HIF3-alpha; hypoxia inducible factor 3, alpha subunit; hypoxia-inducible factor 3-alpha; hypoxia-inducible factor-3 alpha 4; Inhibitory PAS domain protein; IPASHIF3-alpha-1; Member of PAS protein 7; MOP7PAS domain-containing protein 7; PASD7HIF-3A Hif3a|Ipas, MO, MOP7, N, NEPAS, bHLHe1, bHLHe17|hypoxia inducible factor 3, alpha subunit|hypoxia-inducible factor 3-alpha|HIF-3-alpha|HIF3-alpha|HIF3-alpha-1|basic-helix-loop-helix-PAS protein MOP7|hypoxia inducible factor three alpha|inhibitory PAS domain protein|member of PAS protein 7|neonatal and embryonic PAS protein

For more info on Hif3a, check out the Hif3a Infographic

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In this infographic, you will see the following information for Hif3a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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