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Product Info Summary
| SKU: | A09742 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-HIG1 HIGD1A Antibody
SKU/Catalog Number
A09742
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-HIG1 HIGD1A Antibody (Catalog # A09742). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
HIG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-HIG1 HIGD1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09742)
Host
Rabbit
Contents
HIG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HIG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HIG1. The immunogen is located within the first 50 amino acids of HIG1.
Cross-reactivity
HIG1 antibody is predicted to not cross-react with HIG2
Reactive Species
A09742 is reactive to HIGD1A in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
10.1 kDa
Background of HIGD1A
HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG1 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09742 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
HIG1 antibody can be used for detection of HIG1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HIG1 in 293 cell lysate with HIG1 antibody at at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of HIG1 in 293 cells with HIG1 antibody at 2.5 μg/mL.
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Immunofluorescence of HIG1 in 293 cells tissue with HIG1 antibody at 10 μg/mL.
Specific Publications For Anti-HIG1 HIGD1A Antibody (A09742)
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