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Product Info Summary
| SKU: | A15905 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HIG2 HIGD2A Antibody
SKU/Catalog Number
A15905
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-HIG2 HIGD2A Antibody (Catalog # A15905). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
HIG2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-HIG2 HIGD2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15905)
Host
Rabbit
Contents
HIG2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HIG2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human HIG2. The immunogen is located within the last 50 amino acids of HIG2.
Cross-reactivity
At least two isoforms of HIG2 are known to exist; this antibody will detect both isoforms. HIG2 antibody is predicted to not cross-react with HIG1
Reactive Species
A15905 is reactive to HIGD2A in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
11.5 kDa
Background of HIGD2A
HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG2 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15905 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
HIG2 antibody can be used for detection of HIG2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HIG2 in A549 cell lysate with HIG2 antibody at 1 μg/mL.
Specific Publications For Anti-HIG2 HIGD2A Antibody (A15905)
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