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Product Info Summary
| SKU: | A16595-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HIST1H2BG HIST1H2BC Antibody
SKU/Catalog Number
A16595-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HIST1H2BG HIST1H2BC Antibody catalog # A16595-1. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIST1H2BG HIST1H2BC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16595-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human HIST1H2BG(P62807).
Reactive Species
A16595-1 is reactive to HIST1H2BC in Human, Mouse, Rat
Calculated molecular weight
13.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16595-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:100-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using HIST1H2BG antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human oophoroma using Histone H2B type 1-C/E/F/G/I antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Specific Publications For Anti-HIST1H2BG HIST1H2BC Antibody (A16595-1)
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