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- Table of Contents
HDC antibody (A01876-1) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (A01876-1).
Histidine Decarboxylase/HDC was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histidine Decarboxylase/HDC Antibody (A01876-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Histidine Decarboxylase/HDC was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histidine Decarboxylase/HDC Antibody (A01876-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Histidine decarboxylase/HDC Antibody Picoband®
HDC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, IHC, WB |
Tested Applications
HDC antibody was positively detected by WB in:
human HEL whole cell, human K562 whole cell, human Hacat whole cell, human HL-60 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HDC antibody was positively detected by IHC in:
mouse brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Recommend
Mouse
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Histidine decarboxylase/HDC Antibody Picoband®
View all HDC Antibodies
View all HDC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Histidine decarboxylase/HDC recombinant protein (Position: R12-V662).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
74 kDa
Calculated Molecular Weight
74.1 kDa
Background of HDC
HDC(Histidine decarboxylase) is the enzyme that catalyzes the reaction that produces histamine from histidine with the help of vitamin B6. The HDC gene is mapped on 15q21.2. The deduced 662-amino acid protein has a molecular mass of 74,148 Da. The gene contains 12 exons spanning approximately 24 kb. The biogenic amine histamine is an important modulator of numerous physiologic processes, including neurotransmission, gastric acid secretion, and smooth muscle tone. The biosynthesis of histamine from histidine is catalyzed by the enzyme L-histidine decarboxylase. This homodimeric enzyme is a pyridoxal phosphate(PLP)-dependent decarboxylase and is highly specific for its histidine substrate. Ercan-Sencicek et al. noted that animal studies had shown that lack of Hdc in mice results in increased locomotor and stereotypic behaviors, as well as increased anxiety.
Cite This Product
Anti-Histidine decarboxylase/HDC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01876-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
92%
High Homology
Cow
91%
High Homology
Sheep
90%
High Homology
Rabbit
88%
Moderate Homology
Mouse
87%
Moderate Homology
Rat
86%
Moderate Homology
Zebrafish
57%
Low homology
Drosophila
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HDC antibody
Immunohistochemistry (IHC) protocol for HDC antibody
Publications
Application:
Species:
Cooperative response loops enhanced by histamine signaling during tumor-neutrophil crosstalk in pleural mesothelioma
Published: 2026-05-14
Journal: Cell Reports
Application: IHC
Impact Factor: 7.70
Species: Human
PMID: 42139053
The Expression of PTHLH in Human Gastric Mucosa Enterochromaffin-Like Cells
Published: 2010-09-16
Journal: DIGESTIVE DISEASES AND SCIENCES
Application: IHC
Impact Factor: 1.84
Species: Human
PMID: 20844958
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Product Promise
We promise Anti-Histidine decarboxylase/HDC Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01876-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026