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Product Info Summary
| SKU: | M16777-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody
SKU/Catalog Number
M16777-3
BM4311 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody catalog # M16777-3. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16777-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DEB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2A (acetyl K9) Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Reactive Species
M16777-3 is reactive to HIST1H2AB in Human, Mouse, Rat
Observed Molecular Weight
14 kDa
Calculated molecular weight
14.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M16777-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: HeLa cell
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Histone H2A (acetyl K9) expression in HeLa cell lysate treated Trichostatin A.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using Histone H2A (acetyl K9) Antibody.
Specific Publications For Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody (M16777-3)
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