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- Table of Contents
H2AFX antibody (M00241-1) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of H2AFX using anti-H2AFX antibody (M00241-1).
H2AFX was detected in a paraffin-embedded section of human breast hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-H2AFX Antibody (M00241-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
H2AFX was detected in a paraffin-embedded section of human breast hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-H2AFX Antibody (M00241-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Histone H2A.X H2AFX Rabbit Monoclonal Antibody
H2AFX antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
H2AFX antibody was positively detected by WB in:
human Raji whole cell, human MCF-7 whole cell, human U20S whole cell, human SiHa whole cell, rat testis tissue, rat thymus tissue, mouse testis tissue, mouse thymus tissues
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
H2AFX antibody was positively detected by IHC in:
human breast hyperplasia tissue, human testis cancer tissue, mouse testis tissue, rat testis tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
H2AFX antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Histone H2A.X H2AFX Rabbit Monoclonal Antibody
View all H2AFX Antibodies
View all H2AFX Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BIA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2A.X
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
15 kDa
Calculated Molecular Weight
15.1 kDa
Cite This Product
Anti-Histone H2A.X H2AFX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00241-1)
Protocols
Western Blot protocol for H2AFX antibody
Immunohistochemistry (IHC) protocol for H2AFX antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for H2AFX antibody
Publications
Application:
Species:
ASGR1 exacerbates MASLD by inducing hepatocyte senescence via lysosomal dysfunction and HIF-1α stabilization
Published: 2026-08-19
Journal: METABOLISM-CLINICAL AND EXPERIMENTAL
Application: WB
Impact Factor: 16.70
Species: Mouse
PMID: 42617834
Exploring the Role of Nesprin1 in isoproterenol-induced heart failure: Insights into pathogenesis and therapeutic implications
Published: 2026-08-11
Journal: PLoS One
Application: WB,IF
Impact Factor: 2.80
Species: Mouse
PMID: 42579703
PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer
Published: 2025-03-31
Journal: Advanced Science
Application: WB
Impact Factor: 14.10
Species: Human
PMID: 40162859
Review
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Product Promise
We promise Anti-Histone H2A.X H2AFX Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00241-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 22, 2026