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- Table of Contents
HIST1H2AB antibody (M16777-2) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis of Hela cells, using Histone H2A Antibody.
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Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody
HIST1H2AB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
HIST1H2AB antibody was positively detected by WB in:
: human Hela whole cell, human Jurkat whole cell, human 293T whole cell, human LNCAP whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HIST1H2AB antibody was positively detected by IHC in:
human stomach cancer tissue, human pancreas cancer tissue, human ovarian cancer tissue, human esophageal cancer tissue, human lung cancer tissue, human breast cancer tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HIST1H2AB antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Product Information
Product Name
Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody
View all HIST1H2AB Antibodies
View all HIST1H2AB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DFA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2A
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
14 kDa
Calculated Molecular Weight
14.1 kDa
Cite This Product
Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16777-2)
Protocols
Western Blot protocol for HIST1H2AB antibody
Immunohistochemistry (IHC) protocol for HIST1H2AB antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HIST1H2AB antibody
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Product Promise
We promise Anti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M16777-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026