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Product Info Summary
| SKU: | A12594 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Histone H2B H2BFS Antibody
SKU/Catalog Number
A12594
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Histone H2B H2BFS Antibody catalog # A12594. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2B H2BFS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12594)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2B. AA range:10-59
Reactive Species
A12594 is reactive to H2BFS in Human, Monkey, Mouse
Calculated molecular weight
13.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12594 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of COS7 cells using Histone H2B Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Histone H2B H2BFS Antibody (A12594)
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Customer Q&As
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5 Customer Q&As for Anti-Histone H2B H2BFS Antibody
Question
I have a question about product A12594, anti-Histone H2B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12594 anti-Histone H2B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-10
Question
Is this A12594 anti-Histone H2B antibody reactive to the isotypes of H2BFS?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The immunogen of A12594 anti-Histone H2B antibody is Synthesized peptide derived from human Histone H2B around the non-acetylation site of K12. at AA rangle: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Histone H2B antibody A12594. Let me know if you need anything else.
P. Krishna
Verified customer
Asked: 2019-02-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-12
Question
I see that the anti-Histone H2B antibody A12594 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-12-06
Question
We are currently using anti-Histone H2B antibody A12594 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
The anti-Histone H2B antibody (A12594) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-26
