Anti-Histone H2B H2BFS Antibody

H2BFS antibody

Boster Bio Anti-Histone H2B H2BFS Antibody catalog # A12594. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Product Info Summary

SKU: A12594
Size: 100μl
Reactive Species: Human, Monkey, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-Histone H2B H2BFS Antibody

View all H2BFS Antibodies

SKU/Catalog Number

A12594

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Histone H2B H2BFS Antibody catalog # A12594. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone H2B H2BFS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12594)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H2B. AA range:10-59

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12594 is reactive to H2BFS in Human, Monkey, Mouse

Reconstitution

Calculated molecular weight

13944 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12594 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For H2BFS (Source: Uniprot.org, NCBI)

Gene Name

H2BFS

Full Name

Histone H2B type F-S

Weight

13944 MW

Superfamily

histone H2B family

Alternative Names

Histone H2B type F-S;Histone H2B.s;H2B/s;H2BFS;

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on H2BFS, check out the H2BFS Infographic

H2BFS infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for H2BFS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Histone H2B H2BFS Antibody

Question

I have a question about product A12594, anti-Histone H2B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12594 anti-Histone H2B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-10

Question

Is this A12594 anti-Histone H2B antibody reactive to the isotypes of H2BFS?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

The immunogen of A12594 anti-Histone H2B antibody is Synthesized peptide derived from human Histone H2B around the non-acetylation site of K12. at AA rangle: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-06

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Histone H2B antibody A12594. Let me know if you need anything else.

P. Krishna

Verified customer

Asked: 2019-02-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-12

Question

I see that the anti-Histone H2B antibody A12594 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-12-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-12-06

Question

We are currently using anti-Histone H2B antibody A12594 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-09-26

Answer

The anti-Histone H2B antibody (A12594) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-26

Order DetailsPrice
A12594

100uL

$399

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A12594
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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