Product Info Summary
SKU: | A12594 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Monkey, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Histone H2B H2BFS Antibody
SKU/Catalog Number
A12594
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Histone H2B H2BFS Antibody catalog # A12594. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2B H2BFS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12594)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2B. AA range:10-59
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12594 is reactive to H2BFS in Human, Monkey, Mouse
Reconstitution
Calculated molecular weight
13944 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12594 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of COS7 cells using Histone H2B Polyclonal antibody.
Click image to see more details
Western Blot (WB) analysis of specific cells using Histone H2B Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For H2BFS (Source: Uniprot.org, NCBI)
Gene Name
H2BFS
Full Name
Histone H2B type F-S
Weight
13944 MW
Superfamily
histone H2B family
Alternative Names
Histone H2B type F-S;Histone H2B.s;H2B/s;H2BFS;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H2BFS, check out the H2BFS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H2BFS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H2B H2BFS Antibody (A12594)
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No publications found for A12594
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Customer Q&As
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5 Customer Q&As for Anti-Histone H2B H2BFS Antibody
Question
I have a question about product A12594, anti-Histone H2B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12594 anti-Histone H2B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-10
Question
Is this A12594 anti-Histone H2B antibody reactive to the isotypes of H2BFS?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The immunogen of A12594 anti-Histone H2B antibody is Synthesized peptide derived from human Histone H2B around the non-acetylation site of K12. at AA rangle: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Histone H2B antibody A12594. Let me know if you need anything else.
P. Krishna
Verified customer
Asked: 2019-02-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-12
Question
I see that the anti-Histone H2B antibody A12594 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-06
Question
We are currently using anti-Histone H2B antibody A12594 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
The anti-Histone H2B antibody (A12594) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-26