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Product Info Summary
| SKU: | M07286 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Histone H2B (mono methyl R79) HIST2H2BE Rabbit Monoclonal Antibody
SKU/Catalog Number
M07286
BM4324 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H2B (mono methyl R79) HIST2H2BE Rabbit Monoclonal Antibody catalog # M07286. Tested in WB application. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2B (mono methyl R79) HIST2H2BE Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07286)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DFD-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2B (mono methyl R79)
Reactive Species
M07286 is reactive to HIST2H2BE in Human, Mouse
Observed Molecular Weight
14 kDa
Calculated molecular weight
13.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07286 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Histone H2B (mono methyl R79) expression in HeLa cell lysate.
Specific Publications For Anti-Histone H2B (mono methyl R79) HIST2H2BE Rabbit Monoclonal Antibody (M07286)
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Customer Q&As
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5 Customer Q&As for Anti-Histone H2B (mono methyl R79) HIST2H2BE Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody M07286 available?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody M07286 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-13
Question
I was wanting to use your anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
You can see on the product datasheet, M07286 anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-31
Question
Our lab want to know about to test anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody M07286 on mouse brain for research purposes, then I may be interested in using anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody M07286 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
The products we sell, including anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody M07286, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-25
Question
I see that the anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody M07286 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-04-10
Question
We are currently using anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody M07286 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
P. Jackson
Verified customer
Asked: 2015-12-10
Answer
The anti-Histone H2B (mono methyl R79) Rabbit Monoclonal antibody (M07286) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-10
