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- Table of Contents
H3F3A antibody (M06819) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Immunohistochemical analysis of paraffin-embedded mouse stomach, using Histone H3.3 Antibody .
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Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody
H3F3A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
H3F3A antibody was positively detected by IHC in:
mouse stomach tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Mouse
Recommend
Tested
Recommend
Recommend
Human
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody
View all H3F3A Antibodies
View all H3F3A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EBF-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H3.3
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
15 kDa
Calculated Molecular Weight
15.3 kDa
Cite This Product
Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819)
Protocols
Immunohistochemistry (IHC) protocol for H3F3A antibody
Publications
Application:
Species:
SIRT1-mediated deacetylation and activation of MEK/ERK pathway decreased IL-6 in spinal dorsal horn to promote oxycodone tolerance
Published: 2025-07-04
Journal: NEUROPHARMACOLOGY
Application: WB
Impact Factor: 4.60
Species: Mouse
PMID: 40617466
Getah virus nonstructural protein 2 suppresses interferon-beta production by interrupting interferon regulatory factor 3 activation
Published: 2025-06-07
Journal: VETERINARY RESEARCH
Application: WB
Impact Factor: 3.50
Species: Pig
PMID: 40483467
IL-10 upregulates SOCS3 to inhibit type I interferon signaling to promote PoRVA replication in intestinal epithelial cells
Published: 2024-09-23
Journal: VETERINARY MICROBIOLOGY
Application: WB
Impact Factor: 2.40
Species: Pig
PMID: 39332165
METTL14-mediated upregulation of lncRNA HOTAIR represses PP1α expression by promoting H3K4me1 demethylation in oxycodone-treated mice
Published: 2024-07-24
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 39046182
Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein
Published: 2023-08-31
Journal: VETERINARY MICROBIOLOGY
Application: WB
Impact Factor: 3.30
Species: Rat
PMID: 37672899
Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR
Published: 2022-01-22
Journal: VETERINARY MICROBIOLOGY
Application: WB
Impact Factor: 3.25
Species: Porcine
PMID: 35085949
2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity
Published: 2022-01-11
Journal: Cell Reports
Impact Factor: 10.00
Species: Human
PMID: 35021081
Porcine circovirus type 2 infection attenuates the K63-linked ubiquitination of STING to inhibit IFN-β induction via p38-MAPK pathway
Published: 2021-05-04
Journal: VETERINARY MICROBIOLOGY
Application: WB
Impact Factor: 3.29
Species: Pig
PMID: 33984793
Review
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Product Promise
We promise Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M06819
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026