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- Table of Contents
HIST1H3A antibody (M12477-6) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of Histone H3 (mono methyl K18) using anti-Histone H3 (mono methyl K18) antibody (M12477-6).
Histone H3 (mono methyl K18) was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-Histone H3 (mono methyl K18) Antibody (M12477-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Histone H3 (mono methyl K18) was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-Histone H3 (mono methyl K18) Antibody (M12477-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Histone H3 (mono methyl K18) HIST1H3A Rabbit Monoclonal Antibody
HIST1H3A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
HIST1H3A antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human CACO-2 whole cell, human HepG2 whole cell, human PC-12 whole cell, rat C6 whole cell, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HIST1H3A antibody was positively detected by IHC in:
mouse brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HIST1H3A antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Product Information
Product Name
Anti-Histone H3 (mono methyl K18) HIST1H3A Rabbit Monoclonal Antibody
View all HIST1H3A Antibodies
View all HIST1H3A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DFC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H3 (mono methyl K18)
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
15.4 kDa
Cite This Product
Anti-Histone H3 (mono methyl K18) HIST1H3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12477-6)
Protocols
Western Blot protocol for HIST1H3A antibody
Immunohistochemistry (IHC) protocol for HIST1H3A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HIST1H3A antibody
Publications
Application:
Antiproliferative and proapoptotic effects of somatostatin on activated hepatic stellate cells
PMID: 15052685
Role of endoplasmic reticulum stress in apoptosis of differentiated mouse podocytes induced by high glucose
PMID: 24503896
Review
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Product Promise
We promise Anti-Histone H3 (mono methyl K18) HIST1H3A Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M12477-6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 22, 2026