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Product Info Summary
| SKU: | M14495-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H4 (acetyl K16) HIST1H4A Monoclonal Antibody
SKU/Catalog Number
M14495-3
BM4161 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H4 (acetyl K16) HIST1H4A Monoclonal Antibody catalog # M14495-3. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H4 (acetyl K16) HIST1H4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14495-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
COC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H4 (acetyl K16)
Reactive Species
M14495-3 is reactive to HIST1H4A in Human, Mouse, Rat
Observed Molecular Weight
15 kDa
Calculated molecular weight
11.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14495-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human Hela whole cell, human U2OS whole cell, human Jurkat whole cell, human HEL whole cell
IHC: rat lung tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Histone H4 using anti-Histone H4 antibody (M14495-3).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H4 antigen affinity purified monoclonal antibody (M14495-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Histone H4 at approximately 15 kDa. The expected band size for Histone H4 is at 11 kDa.
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Immunohistochemical analysis of paraffin-embedded rat lung, using Histone H4 (acetyl K16) Antibody.
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Immunofluorescent analysis of HeLa cells treated with TSA, using Histone H4 (acetyl K16) Antibody .
Specific Publications For Anti-Histone H4 (acetyl K16) HIST1H4A Monoclonal Antibody (M14495-3)
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