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Product Info Summary
| SKU: | M14495-4 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody
SKU/Catalog Number
M14495-4
BM4189 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody catalog # M14495-4. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14495-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CCA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H4 (acetyl K5) Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Reactive Species
M14495-4 is reactive to HIST1H4A in Human, Mouse, Rat
Observed Molecular Weight
11 kDa
Calculated molecular weight
11.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14495-4 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: Untreated HeLa HeLa cell, TSA treated HeLa cell
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of Histone H4 (acetyl K5) expression in (1) Untreated HeLa HeLa cell lysate; (2) TSA treated HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon, using Histone H4 (acetyl K5) Antibody.
Specific Publications For Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody (M14495-4)
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