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- Table of Contents
HIST1H4A antibody (M14495-4) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunohistochemical analysis of paraffin-embedded human colon, using Histone H4 (acetyl K5) Antibody.
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Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody
HIST1H4A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
HIST1H4A antibody was positively detected by WB in:
Untreated HeLa HeLa cell, TSA treated HeLa cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HIST1H4A antibody was positively detected by IHC in:
human colon tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
ICC
IF
IP
Human
Tested
Recommend
Recommend
Recommend
Mouse
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody
View all HIST1H4A Antibodies
View all HIST1H4A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CCA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H4 (acetyl K5) Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
11 kDa
Calculated Molecular Weight
11.4 kDa
Cite This Product
Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14495-4)
Protocols
Western Blot protocol for HIST1H4A antibody
Immunohistochemistry (IHC) protocol for HIST1H4A antibody
Publications
Application:
Species:
Paeonol inhibits chronic constriction injury-induced astrocytic activation and neuroinflammation in rats via the HDAC/miR-15a pathway
Published: 2022-09-05
Journal: DRUG DEVELOPMENT RESEARCH
Application: WB
Impact Factor: 5.00
Species: Rat
PMID: 36063531
Review
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Product Promise
We promise Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M14495-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026