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Product Info Summary
| SKU: | A00194-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-HLA-B Antibody (N-term)
SKU/Catalog Number
A00194-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-HLA-B Antibody (N-term) (Catalog # A00194-1). Tested in IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HLA-B Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00194-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 75-107 amino acids from the N-terminal region of human HLA-B.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00194-1 is reactive to HLA-B in Human
Calculated molecular weight
40.5 kDa
Background of HLA-B
Involved in the presentation of foreign antigens to the immune system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00194-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Ramos whole cell lysate
Lane 5: human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded H. tonsil section using HLA-B Antibody (N-term). A00194-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. spleen section using HLA-B Antibody (N-term). A00194-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded R. spleen section using HLA-B Antibody (N-term). A00194-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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A00194-1 staining HLA-B in human tonsil tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-HLA-B Antibody (N-term) (A00194-1)
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