Anti-HLA DMB/HLA-DMB Antibody

Product Name

Anti-HLA DMB/HLA-DMB Antibody

View all HLA DMB Antibodies

SKU/Catalog Number

PA1788

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLA DMB/HLA-DMB Antibody catalog # PA1788. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA DMB/HLA-DMB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1788)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human HLA DMB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1788 is reactive to HLA-DMB in Human

Applications

PA1788 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

28.943kDa

Background of HLA DMB

HLA-DMB (major histocompatibility complex, class II, DM beta), also known as D6S221E, RING7, HLA-DM histocompatibility type, beta chain, HLADMB or RING7, is a protein that in humans is encoded by the HLA-DMB gene. The HLA-DMB gene is mapped on 6p21.32. HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. HLA-DMA and -DMB appear to encode subunits of a functional heterodimer that is critical in the pathway of class II antigen presentation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-HLA DMB antibody, PA1788, Western blotting
All lanes: Anti HLA DMB (PA1788) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD

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Anti-HLA DMB antibody, PA1788, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Gene/Protein Information For HLA-DMB (Source: Uniprot.org, NCBI)

Alternative Names

class II histocompatibility antigen, M beta chain; D6S221E; DMB; major histocompatibility complex, class II, DM beta; MHC class II antigen DMB; MHC class II antigen HLA-DM beta chain; MHC class II HLA-DMB; Really interesting new gene 7 protein; RING7HLA class II histocompatibility antigen, DM beta chain HLA-DMB D6S221E, RING7 major histocompatibility complex, class II, DM beta HLA class II histocompatibility antigen, DM beta chain|MHC class II HLA-DMB|MHC class II antigen DMB|MHC class II antigen HLA-DM beta chain|class II histocompatibility antigen, M beta chain|really interesting new gene 7 protein

For more info on HLA-DMB, check out the HLA-DMB Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HLA-DMB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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