Anti-HLA-DPB1 Antibody Picoband™

Product Name

Anti-HLA-DPB1 Antibody Picoband™

View all HLA DPB1 Antibodies

SKU/Catalog Number

A00487

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLA-DPB1 Antibody Picoband™ catalog # A00487. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA-DPB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00487)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human HLA-DPB1 recombinant protein (Position: R30-A258).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00487 is reactive to HLA-DPB1 in Human

Applications

A00487 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

29.159kDa

Background of HLA DPB1

HLA class II histocompatibility antigen, DP (W2) beta chain is a protein that in humans is encoded by the HLA-DPB1 gene. HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry, 2-5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HLA-DPB1 using anti-HLA-DPB1 antibody (A00487).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DPB1 antigen affinity purified polyclonal antibody (Catalog # A00487) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DPB1 at approximately 29 kDa. The expected band size for HLA-DPB1 is at 29 kDa.

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Figure 2. IHC analysis of HLA-DPB1 using anti-HLA-DPB1 antibody (A00487).
HLA-DPB1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HLA-DPB1 Antibody (A00487) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For HLA-DPB1 (Source: Uniprot.org, NCBI)

Alternative Names

DP beta 1 chain; DPB1; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA DP14-beta chain; HLA-DP histocompatibility type, beta-1 subunit; HLA-DP1B; HLA-DPB; major histocompatibility complex class II HLA DPB1 protein; major histocompatibility complex, class II, DP beta 1; MHC class II antigen beta chain; MHC class II antigen DP beta 1 chain; MHC class II antigen DPB1; MHC class II antigen DPbeta1; MHC class II HLA-DP-beta; MHC HLA DPB1 HLA-DPB1 DPB1, HLA-DP, HLA-DP1B, HLA-DPB major histocompatibility complex, class II, DP beta 1 HLA class II histocompatibility antigen, DP beta 1 chain|HLA class II histocompatibility antigen, DP(W4) beta chain|HLA-DP histocompatibility type, beta-1 subunit|MHC HLA DPB1|MHC class II HLA-DP-beta-1|MHC class II antigen DPB1

For more info on HLA-DPB1, check out the HLA-DPB1 Infographic

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In this infographic, you will see the following information for HLA-DPB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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