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Product Info Summary
| SKU: | M00232-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-HLA-DQA1 Monoclonal Antibody
SKU/Catalog Number
M00232-1
BM5268 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HLA-DQA1 Monoclonal Antibody catalog # M00232-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA-DQA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00232-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACEC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HLA-DQA1 HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins.
Reactive Species
M00232-1 is reactive to HLA-DQA1 in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
27.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00232-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human spleen
Validation Images & Assay Conditions
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Western blot analysis of HLA-DQA1 expression in human spleen lysate.
Specific Publications For Anti-HLA-DQA1 Monoclonal Antibody (M00232-1)
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Customer Q&As
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4 Customer Q&As for Anti-HLA-DQA1 Monoclonal Antibody
Question
Will M00232-1 anti-HLA-DQA1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can see on the product datasheet, M00232-1 anti-HLA-DQA1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-03
Question
Do you have a BSA free version of anti-HLA-DQA1 Monoclonal antibody M00232-1 available?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HLA-DQA1 Monoclonal antibody M00232-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-01
Question
Does anti-HLA-DQA1 Monoclonal antibody M00232-1 work for WB with b-cell?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
According to the expression profile of b-cell, HLA-DQA1 is highly expressed in b-cell. So, it is likely that anti-HLA-DQA1 Monoclonal antibody M00232-1 will work for WB with b-cell.
Boster Scientific Support
Answered: 2019-05-15
Question
We are currently using anti-HLA-DQA1 Monoclonal antibody M00232-1 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
The anti-HLA-DQA1 Monoclonal antibody (M00232-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-12
