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Product Info Summary
|Application:||IF, IHC, ICC, WB|
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Anti-HLA-Drb1 Monoclonal Antibody
Boster Bio Anti-HLA-Drb1 Monoclonal Antibody catalog # M00568-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA-Drb1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00568-1)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human HLA-Drb1 Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M00568-1 is reactive to HLA-DRB1 in Human
M00568-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HLA-DRB1 using anti-HLA-DRB1 antibody (M00568-1) in Ramos cell lysate.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DRB1 antigen affinity purified polyclonal antibody (Catalog # M00568-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Gene/Protein Information For HLA-DRB1 (Source: Uniprot.Org, NCBI)
HLA class II histocompatibility antigen, DRB1 beta chain
Clone P2-beta-3; DR1; DR-1; DR12; DR-12; DR13; DR-13; DR14; DR-14; DR16; DR-16; DR4; DR-4; DR5; DR-5; DR7; DR-7; DR8; DR-8; DR9; DR-9; DRB1; DRw10; DRw11; DRw8; DW2.2/DR2.2; FLJ75017; FLJ76359; HLA class II antigen beta chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA-DR1B; HLA-DRB; HLA-DRB1*; HLA-DRB2; HLA-DR-beta 1; human leucocyte antigen DRB1; leucocyte antigen DR beta 1 chain; leucocyte antigen DRB1; lymphocyte antigen DRB1; major histocompatibility complex, class II, DR beta 1; MHC class II antigen DRB1*1; MHC class II antigen DRB1*10; MHC class II antigen DRB1*11; MH HLA-DRB1 DRB1, HLA-DR1B, HLA-DRB, SS1 major histocompatibility complex, class II, DR beta 1 major histocompatibility complex, class II, DR beta 1 precursor|HLA class II histocompatibility antigen, DR-1 beta chain|MHC class II HLA-DR beta 1 chain|human leucocyte antigen DRB1|lymphocyte antigen DRB1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on HLA-DRB1, check out the HLA-DRB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HLA-DRB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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