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Product Info Summary
| SKU: | A06177-2 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Bovine, Dog, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-HLX1 Monoclonal Antibody
SKU/Catalog Number
A06177-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HLX1 Monoclonal Antibody catalog # A06177-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Bovine, Dog, Pig.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLX1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06177-2)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Isotype
IgG
Immunogen
Purified recombinant human HLX1 (C-terminus) protein fragments expressed in E.coli.
Reactive Species
A06177-2 is reactive to HLX in Bovine, Dog, Human, Mouse, Pig, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
50.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06177-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Validation Images & Assay Conditions
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Western Blot analysis using HLX1 Monoclonal Antibody against Mouse pancreas cell lysate.
Specific Publications For Anti-HLX1 Monoclonal Antibody (A06177-2)
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Customer Q&As
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6 Customer Q&As for Anti-HLX1 Monoclonal Antibody
Question
Will anti-HLX1 Monoclonal antibody A06177-2 work on horse WB with b-cell?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Our lab technicians have not tested anti-HLX1 Monoclonal antibody A06177-2 on horse. You can run a BLAST between horse and the immunogen sequence of anti-HLX1 Monoclonal antibody A06177-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse b-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-28
Question
Is this A06177-2 anti-HLX1 Monoclonal antibody reactive to the isotypes of HLX?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The immunogen of A06177-2 anti-HLX1 Monoclonal antibody is Purified recombinant human HLX1 (C-terminal) protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-04
Question
I was wanting to use your anti-HLX1 Monoclonal antibody for WB for mouse bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse bone marrow identification?
N. Wu
Verified customer
Asked: 2018-09-04
Answer
As indicated on the product datasheet, A06177-2 anti-HLX1 Monoclonal antibody has been tested for WB on bovine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-04
Question
Is there a BSA free version of anti-HLX1 Monoclonal antibody A06177-2 available?
B. Patel
Verified customer
Asked: 2016-05-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HLX1 Monoclonal antibody A06177-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-27
Question
Here is the WB image, lot number and protocol we used for bone marrow using anti-HLX1 Monoclonal antibody A06177-2. Please let me know if you require anything else.
J. Parker
Verified customer
Asked: 2016-01-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-12
Question
Is a blocking peptide available for product anti-HLX1 Monoclonal antibody (A06177-2)?
J. Parker
Verified customer
Asked: 2015-03-10
Answer
We do provide the blocking peptide for product anti-HLX1 Monoclonal antibody (A06177-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-03-10
