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Product Info Summary
| SKU: | A00436-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-HMGA2 Antibody (C-term)
SKU/Catalog Number
A00436-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-HMGA2 Antibody (C-term) (Catalog # A00436-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HMGA2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00436-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HMGA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the C-terminal region of human HMGA2.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00436-1 is reactive to HMGA2 in Human
Calculated molecular weight
11.8 kDa
Background of HMGA2
This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00436-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
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HMGA2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the HMGA2 antibody detected the HMGA2 protein (arrow).
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HMGA2 Antibody (C-term) (Cat. #A00436-1) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-HMGA2 Antibody (C-term) (A00436-1)
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